This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wallnöfer, P. R. Articles by Bader, J. Search for Related Content PubMed Articles by Wallnöfer, P. R. Articles by Bader, J. Agricola Articles by Wallnöfer, P. R. Articles by Bader, J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 May; 19(5): 714-717
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Degradation of Urea Herbicides by Cell-Free Extracts of Bacillus sphaericus

Bayerische Landesanstalt für Bodenkultur, Pflanzenbau und Pflanzenschutz, Munich, Germany

ABSTRACT

N'-methoxy phenylurea herbicides are degraded by induced cells of Bacillus sphaericus ATCC 12123 by liberating carbon dioxide from the ureido portion of the molecule and leaving the corresponding aniline moieties. Cell-free extracts of B. sphaericus inactivate these herbicides in the same way as reported for whole cells. A 6.6-fold purification of the crude extract was achieved by a combination of salt fractionation with ammonium sulfate and column chromatography on diethyl-aminoethyl cellulose.