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Appl Environ Microbiol. 1970 May; 19(5): 785-790
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Method for Extracting Viral Hemagglutination-Inhibiting Antibodies from the Nonspecific Inhibitors of Serum

Department of Virus Diseases, Walter Reed Army Institute of Research, and Department of Pediatrics, Georgetown University Medical Center, Washington, D. C. 20007

ABSTRACT

Various methods are used to remove nonspecific inhibitors from sera before titering viral hemagglutination-inhibiting antibodies. These methods have several undesirable features; some are tedious and time-consuming, some remove antibody along with nonspecific inhibitors, and different techniques are usually required to remove the nonspecific inhibitors for different viruses. This communication describes a single method that uses diethylaminoethyl-Sephadex to extract the immunoglobulin G antibodies for several viruses from nonspecific inhibitors. The procedure is fast, simple to perform, and removed the nonspecific inhibitors for influenza, Western equine encephalitis, dengue-2, and rubella viruses.

¹ Present address: Department of Pediatrics, University of Florida College of Medicine, Gainesville, Fla. 32601.