This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cho, C. T. Articles by Wenner, H. A. Search for Related Content PubMed Articles by Cho, C. T. Articles by Wenner, H. A. Agricola Articles by Cho, C. T. Articles by Wenner, H. A.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 May; 19(5): 791-794
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Viremia and Virus Measurements of Rabbit Pox in CV-1 Cells

Section of Virus Research, Department of Pediatrics, University of Kansas Medical Center, Kansas City, Kansas 66103

ABSTRACT

Rabbit pox virus (RPV) produced cytopathic effect (CPE) in five types of cells grown in tissue cultures. The CPE on CV-1 cells was characterized by cell fusion and lysis. The CV-1 line is a useful and sensitive cell culture for measuring concentrations of RPV in blood and tissues of infected rabbits. Viremia was detected between the 2nd and 4th days after parenteral infection. By the 6th and 7th days, the concentration of RPV in various tissues ranged from 10^5-3 to 10^8-5 TCID₅₀/g. Cross-reactivity was demonstrated by the fluorescent-antibody technique between rabbit pox, vaccinia, and monkey pox viruses.

¹ Fellow in Infectious Diseases, Department of Pediatrics, University of Kansas School of Medicine, Kansas City, Kan. 66103.

² Present address: The Children's Mercy Hospital of the University of Missouri, Kansas City, Mo. 64106.