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Appl Environ Microbiol. 1970 May; 19(5): 818-822
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Somatic Antigens of Streptococcus Group E

I. Comparison of Extraction Techniques¹

Department of Veterinary Microbiology, Pathology, and Public Health, School of Veterinary Science and Medicine, Purdue University, Lafayette, Indiana 47907

ABSTRACT

Eleven Streptococcus group E strains, representing serotypes I, II, III, IV, V, and "untypable" isolates, were extracted by formamide, trichloroacetic acid, and hydrochloric acid under various conditions in an effort to determine the best method for recovering maximum amounts of group and type antigens. The group antigen was found to be relatively stable, and adequate amounts for identification purposes were recovered by a wide spectrum of conditions. Type-specific antigens were relatively labile, and were destroyed at low pH in acid hydrolysis or by prolonged heating in formamide hydrolysis. The best single procedure for recovering both type and group antigens from Streptococcus group E was formamide hydrolysis for 30 min at 180 C.

² Present address: Amdal Co., P.O. Box 98, Berlin, Md. 21811.

¹ Published as journal paper 3943 by the Purdue University Agricultural Experiment Station.