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Appl Environ Microbiol. 1970 May; 19(5): 823-829
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Somatic Antigens of Streptococcus Group E

II. Separation and a Partial Physicochemical Characterization¹

Department of Veterinary Microbiology, Pathology, and Public Health, School of Veterinary Science and Medicine, Purdue University, Lafayette, Indiana 47907

ABSTRACT

Antigenic extracts of Streptococcus group E (SGE) were subjected to fractional ethanol precipitation, block (preparative) electrophoresis, and gel filtration for the purpose of separating the type antigen from the group antigen. Ethanol precipitation was ineffective in separating the substances. Block electrophoresis yielded serologically pure group antigen and a mixture of type and group antigen. Serologically pure type antigen was obtained by gel filtration. In some cases, pure group antigen was also recovered; in others, it was contaminated with type antigen. Gel filtration column effluents of antigenic extracts of SGE serotypes, I, II, III, IV, V and "untypable" isolates, collected from the region in which type antigen was eluted, were studied by paper chromatography and infrared spectrophotometry in an effort to develop a nonserological means of detecting type antigen. Hydrolysates of type antigens or suspect type antigens of serotypes I through V contained L-rhamnose, D-glucose, and several unidentified substances. D-Galactose also was present in hydrolysates of serotypes III and V. Untypable isolates and negative controls contained traces of D-glucose only. The data suggested that serotypes I through V contained a type antigen and that the untypable isolates were devoid of type antigen. Infrared absorbance spectra seemed to support the paper chromatography data.

² Present address: Amdal Co., P.O. Box 98, Berlin, Md. 21811.

¹ Published as journal paper 3944 by the Purdue University Agricultural Experiment Station.