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Appl Environ Microbiol. 1970 July; 20(1): 78-84
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Characterization of Clostridia by Gas Chromatography: Differentiation of Species by Trimethylsilyl Derivatives of Whole-Cell Hydrolysates

Bacteriology Section, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

Trimethylsilyl (TMS) derivatives prepared from whole-cell hydrolysates of 36 strains, representing 10 species of Clostridium were examined by gas-liquid chromatography (GLC). The TMS profile of each species contained a group of peaks which characterized the species. Variation among strains within a species was much lower than variation between species. Some of the closely related clostridia could be differentiated by comparing their TMS profiles. Strains of Clostridium botulinum were distinguished from C. sporogenes on the basis of the ratio of two GLC peaks which corresponded to arabinose and glucose. A peak with a retention time identical to that of mannose was present in all C. bifermentans strains but was absent in those of C. sordellii.