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Appl Environ Microbiol. 1970 September; 20(3): 416-420
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Use of Acetone-Dried Vaccines for Preparing Capsular Antisera Against the Klebsiella Group and the Lyophilization of Klebsiella Cultures

Biological Reagents Section, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

Capsular antisera against the 72 types of Klebsiella have been prepared in rabbits by using an acetone-dried vaccine. It was shown that encapsulated cells dried with acetone and ground to a fine powder with a mortar and pestle retain their capsules. Antisera obtained from rabbits vaccinated with these vaccines had quellung titers ranging from 1:16 to 1:64. The dried vaccine was stable after storage for over 2 years at room temperature. Encapsulated cultures lyophilized in the presence of 5% sucrose, 5% sodium glutamate, and 5% polyvinylpyrrolidone remained viable and retained their capsules after storage for 10 months at room temperature.