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Appl Environ Microbiol. 1970 October; 20(4): 555-557
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Ester Production by Pseudomonas fragi IV. Demonstration of Esterase Activity ¹

Department of Food Science and Technology, Oregon State University, Coravllis, Oregon 97331

ABSTRACT

Assay of the esterase activity of sonically treated cell-free extracts, whole cell suspensions, and supernatant fluid of Pseudomonas fragi cultures with a differential respirometer revealed that the esterases were intracellular. Polyacrylamide-gel electrophoresis demonstrated six bands of esterase activity, which revealed substrate specificity differences. Band 1 exhibited slow mobility, bands 2, 3, and 4 moderate mobility, and bands 5 and 6 rapid mobility. Six bands were active with -naphthyl acetate, four bands with -naphthyl propionate, and 5 bands with naphthyl butyrate. These esterases appeared to be more active with aromatic esters than with aliphatic esters.

² Present address: Food Technology Research Center, Libbey, McNeill and Libbey, Chicago, Ill. 60643.

³ Present address: Department of Food Science, University [ill] Wisconsin, Madison, Wis. 53706.

¹ Technical Paper no. 2877, Oregon Agricultural Experiment Station.