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Appl Environ Microbiol. 1970 November; 20(5): 667-671
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Continuous-Flow Ultracentrifugation of Canine Distemper Virus and Infectious Canine Hepatitis Virus

Departments of Biochemistry and Obstetrics and Gynecology, College of Medicine, University of Nebraska, Omaha, Nebraska 68105

ABSTRACT

The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.