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Appl Environ Microbiol. 1970 November; 20(5): 687-692
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Sugar Substrates for L-Lysine Fermentation by Ustilago maydis

Facultad de Ciencias, Universidad Central de Venezuela, Apartado 59097, Caracas 104, Venezuela

ABSTRACT

The extracellular production of L-lysine in media with cane sugar, blackstrap molasses, or clarified sugar-cane juice by a previously obtained mutant of Ustilago maydis was studied. Enzymatically inverted clarified juice (medium J-3) gave 2.9 g of lysine per liter under the following conditions: inoculum, 5%; pH 5.8; temperature, 30 C; K_La in the fermentors, 0.41 mmoles of O₂ per liter per min; fermentation time, 72 hr. The concentrate, obtained by direct evaporation and drying of the fermentation broth, could be used as a possible feed supplement because of its amino-acid and vitamin content.