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Appl Environ Microbiol. 1970 November; 20(5): 737-741
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Effect of Bile and Desoxycholate on Gram-Negative Anaerobic Bacteria

Kaoru Shimada1, Vera L. Sutter and Sydney M. Finegold

Department of Medicine and Anaerobic Bacteriology Laboratory, Wadsworth Hospital, Veterans Administration, Los Angeles, California 90073
Department of Medicine, UCLA School of Medicine, Los Angeles, California 90073

ABSTRACT

The bile tests for characterizing gram-negative anaerobic bacilli were reevaluated in prereduced anaerobically sterilized peptone-yeast-glucose broth, in thioglycollate broth, and on blood agar plates. Blood agar plates were unsatisfactory. The combination of 20% bile with 0.1% desoxycholate inhibited Fusobacterium, Bacteroides melaninogenicus, and B. oralis and sometimes Sphaerophorus necrophorus, but not B. fragilis or other Sphaerophorus species studied. Ten per cent bile with 0.05% desoxycholate was less satisfactory. There was no significant difference between fresh and commercial powdered bile. Desoxycholate (0.1% in thioglycollate broth) inhibited B. fragilis, Fusobacterium, B. melaninogenicus, B. oralis, and S. necrophorus, but not S. varius or S. mortiferus/S. ridiculosus. The bile and desoxycholate tests are simple to perform and helpful for characterization and classification of gram-negative anaerobic bacilli.

FOOTNOTES

1 Present address: The First Department of Medicine, School of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.

Appl Environ Microbiol. 1970 November; 20(5): 737-741
Copyright © 1970 American Society for Microbiology. All Rights Reserved.





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