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Appl Environ Microbiol. 1970 December; 20(6): 861-865
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Rapid Detection of Small Numbers of Airborne Bacteria by a Membrane Filter Fluorescent-Antibody Technique

Institute of Veterinary Bacteriology, University of Berne, Berne, Switzerland

ABSTRACT

Bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously described by Danielsson. Calibrated air samples from Serratia marcescens aerosols were drawn through a Millipore aerosol filter holder fitted out with a nonfluorescent membrane filter (type HAB 047). With the aid of a high-power incident-light ultraviolet microscope, the individual bacterial cells, trapped on the filter, could be inspected after staining with appropriate conjugate on the filter surface.

¹ Present address: Eidg. Technische Hochschule, Zürich-Hönggerberg Institut für Molekularbiologie und Biophysik, Zurich, Switzerland.