This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Goepfert, J. M. Articles by Hicks, R. Search for Related Content PubMed Articles by Goepfert, J. M. Articles by Hicks, R. Agricola Articles by Goepfert, J. M. Articles by Hicks, R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 December; 20(6): 977-983
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

One-Day Fluorescent-Antibody Procedure for Detecting Salmonellae in Frozen and Dried Foods ¹

Food Research Institute and Department of Microbiology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The indirect fluorescent-antibody technique was used to examine 422 food samples for the presence of salmonellae. A cultural phase involving a 16-hr preenrichment in buffered nutrient broth-milk medium followed by a 4- to 5-hr subculture into fresh medium of the same composition was evaluated. This procedure yielded a sufficient population of salmonellae so that no false-negative results were obtained. Of the 31 false-positives obtained, 12 samples yielded positive cultural results upon extensive subculture of the original enrichment broths. Yeast cells and both vegetative and spore forms of bacilli were observed to fluoresce when stained with anti-Salmonella serum. Efforts to ascertain the cause of these cross-reactions and several alternate explanations are discussed.

¹ Published with the approval of the Director of the Research Division, College of Agricultural and Life Sciences.