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Appl Environ Microbiol. 1971 March; 21(3): 420-425
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Comparison of Animal Sera for Suitability in Coagulase Testing 1

D. S. Orth2, L. R. Chugg and A. W. Anderson

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331

ABSTRACT

The sera of several animals were examined for suitability in coagulase testing. The assay for coagulase-reacting factor (CRF) activities of the whole sera indicated the following relative concentrations of CRF: human > pig > rabbit > horse > bovine, chicken, and lamb. Human, pig, and rabbit sera had adequate amounts of CRF for coagulase testing. The plasmin activities of the different sera, arranged from the strongest to the weakest, were as follows: rabbit > human > lamb > horse > bovine, chicken, and pig. Fibrinolysis was observed when rabbit, human, lamb, or horse sera were incorporated into coagulase test agars. Pig serum was superior to the other sera for use in the plate test for coagulase since it had adequate amounts of CRF and the plasminogen-plasmin system was not activated by staphylokinase or staphylococcal Müller factor. Heparinized pig plasma was more suitable than citrated pig plasma since citrate interfered with the growth of Staphylococcus aureus, and the use of heparinized plasma prevented false-positive coagulase reactions due to citrate utilization.

FOOTNOTES

2 Present address: The Procter & Gamble Co., Miami Valley Laboratories, Cincinnati, Ohio 45239.

1 Technical Paper no. 2918, Oregon Agricultural Experiment Station, Oregon State University, Corvallis, Ore. 97331.

Appl Environ Microbiol. 1971 March; 21(3): 420-425
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Copyright © 1971 by the American Society for Microbiology. All rights reserved.