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Appl Environ Microbiol. 1971 March; 21(3): 470-475
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Analysis of Clostridium botulinum Toxigenic Types A, B, and E for Fatty and Carbohydrate Content ¹

Food and Drug Administration, Dallas District, Dallas, Texas 75204
The University of Texas, Southwestern Medical School, Dallas, Texas 75235

ABSTRACT

Lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic KOH. Gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of Clostridium botulinum toxigenic types "A," "B," and "E." C. perfringens type "A" and Escherichia coli strain "B" were also studied in a similar manner and were found to give pattern profiles which were distinct even from those obtained for the C. botulinum microorganisms. Amino sugar content of the five microorganisms was determined by using a Beckman amino acid analyzer. The molar ratio of glucosamine to that of galactosamine was found to be of further assistance in distinguishing the individual microorganisms.

¹ A preliminary report of this investigation was presented before the 69th Meeting of The American Society for Microbiology, Miami Beach, Fla., 4-9 May, 1969.