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Appl Environ Microbiol. 1971 March; 21(3): 520-526
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Factors Affecting Phosphate Uptake by Aerobacter aerogenes in a System Relating Cell Numbers to 32P Uptake 1

Lloyd A. White and Robert A. MacLeod

Defence Research Establishment Suffield, Defence Research Board of Canada, Ralston, Alberta
Department of Microbiology, Macdonald College of McGill University, Macdonald College, Quebec, Canada

ABSTRACT

The uptake of phosphate, from media limited in this ion, by resting cells of Aerobacter aerogenes has been investigated and shown to be dependent upon several factors. An incubation medium composed of 10–2M K+, 5 x 10–3M Mg2+, 1 mg of glucose per ml, and 1 µCi of 32PO43– per ml, buffered at pH 6.55 with 0.05 MN-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid (HEPES), was found to stimulate optimum accumulation of 32P-orthophosphate. The temperature of incubation, incubation time, the concentration of unlabeled orthophosphate, as well as arsenate, and several metabolic inhibitors were found to affect the accumulation. The labeled cells were collected on a membrane filter, which had been previously boiled in glass-distilled water, for measurement of the radioactivity accumulated. Under optimum conditions, as few as 20,000 cells were capable of accumulating detectable amounts of 32P-orthophosphate in 1 hr of incubation.

FOOTNOTES

1 Part of a dissertation submitted by the senior author to the Department of Microbiology, Macdonald College of McGill University, in partial fulfillment of the requirements for the Ph.D. degree. Issued as Suffield Technical Paper no. 364.

Appl Environ Microbiol. 1971 March; 21(3): 520-526
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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