This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ubertini, T. Articles by Noronha, F. Search for Related Content PubMed Articles by Ubertini, T. Articles by Noronha, F. Agricola Articles by Ubertini, T. Articles by Noronha, F.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 March; 21(3): 534-538
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Use of Horseradish Peroxidase-Labeled Antibody for Light and Electron Microscope Localization of Reovirus Antigen

Departments of Pathology and Large Animal Medicine, New York State Veterinary College, Cornell University, Ithaca, New York 14850

ABSTRACT

Horseradish peroxidase-labeled antibody was used for light and electron microscopic localization of reovirus antigen in tissue culture. Reaction product in infected cells was easily detected in the cytoplasm, and the procedure was as sensitive as the fluorescent-antibody technique. At the electron microscopic level, infected and enzyme-labeled antibody-treated cells showed accumulations of reaction product at the sites of viral replication and around the viral particles. Reaction product was not detected in unstained infected cells, in stained uninfected cells, or in cells infected with an unrelated virus.