This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lachica, R. V. F. Articles by Hoeprich, P. D. Search for Related Content PubMed Articles by Lachica, R. V. F. Articles by Hoeprich, P. D. Agricola Articles by Lachica, R. V. F. Articles by Hoeprich, P. D.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 April; 21(4): 585-587
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Metachromatic Agar-Diffusion Methods for Detecting Staphylococcal Nuclease Activity

Department of Microbiology, Weber State College, Ogden, Utah
Section of Infectious and Immunologic Diseases, Department of Internal Medicine, School of Medicine, University of California, Davis, California 95616
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis, California 95616

ABSTRACT

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 µg/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

¹ Present address: Section of Infectious and Immunologic Diseases, Department of Internal Medicine, School of Medicine, University of California, Davis, Calif.