This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Barber, T. L. Articles by Fabricant, J. Search for Related Content PubMed Articles by Barber, T. L. Articles by Fabricant, J. Agricola Articles by Barber, T. L. Articles by Fabricant, J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 April; 21(4): 594-599
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification ¹

Department of Avian Diseases, New York State Veterinary College, Cornell University, Ithaca, New York 14850

ABSTRACT

Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in a pH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.

² Present address: Animal Disease Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Denver Federal Center, Bldg. 45-B, Denver, Colo. 80225.

Submitted by the senior author to Cornell University, Ithaca, N.Y., in partial fulfillment of the requirements for the Ph.D. degree.