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Appl Environ Microbiol. 1971 April; 21(4): 611-613
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Methods for Accelerating the Fluorescent-Antibody Test for Rabies Diagnosis ¹

Pan American Zoonoses Center, Pan American Health Organization, P.O. Box 23, Ramos Mejía, Buenos Aires, Argentina

ABSTRACT

The time required to perform the fluorescent-antibody test for rabies was reduced by eliminating acetone fixation of the brain impressions and by incubating the conjugate-impression reaction at room temperature for only 10 min. Elimination of the preliminary acetone fixation had no effect on the diagnosis of impression smears from 246 mammalian brains by immunofluorescence. Staining at 37 C for 30 min and staining at room temperature for 10 min were found to be equally effective in the examination of impression smears from 161 brain samples. The procedure, as modified, shortens the time required for the diagnosis of rabies by immunofluorescence from about 5.5 hr to approximately 45 min.

² Holder of a PAHO/WHO training fellowship. Present address: Ministerio de Salubridad Pública, San José, Costa Rica.

A preliminary report on this study was presented at the II Jornadas Argentinas de Microbiología, 22-26 November 1970, Buenos Aires, Argentina.