This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hartwell, W. V. Articles by Berquist, K. R. Search for Related Content PubMed Articles by Hartwell, W. V. Articles by Berquist, K. R. Agricola Articles by Hartwell, W. V. Articles by Berquist, K. R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 April; 21(4): 623-625
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Sensitive Matrix Gel Diffusion Test for the Detection of Australia Antigen

Disease Investigations Section, Phoenix Laboratories, Ecological Investigations Program, Center for Disease Control, Phoenix, Arizona 85014

ABSTRACT

A matrix gel diffusion (MGD) procedure with a sensitivity comparable to the complement fixation test (CF) has been developed for detecting Australia antigen in serum. The test utilizes a thin layer of agar (0.1 mm) with an applied plastic matrix. Reactants are introduced directly onto the surface of the agar through wells in the plastic matrix. End points obtained by CF with a panel of 11 sera varied from 1:8 to 1:512. When these sera were tested by MGD, end points for detection of antigen were within one dilution of that obtained by CF.