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Appl Environ Microbiol. 1971 April; 21(4): 718-722
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Production and Evaluation of a Purified Adenovirus Group-Specific (Hexon) Antigen for Use in the Diagnostic Complement Fixation Test

U.S. Public Health Service, Center for Disease Control, Atlanta, Georgia 30333

ABSTRACT

A simple procedure for the production of large volumes of purified adenovirus group-specific complement-fixing (CF) (hexon) antigen by selective adsorption to and elution from CaHPO₄ is described. Results of immunodiffusion tests, electrophoresis, electron microscopy, and tests for hemagglutination and infectivity indicate that the purified antigen consisted of a single virus component (hexon). The purified product contained little host materials. Unlike the crude virus harvest usually employed for serodiagnostic CF tests, the purified antigen demonstrated no anticomplementary activity and did not develop such activity during storage. The purified antigen was equal to or slightly more sensitive than crude virus harvests for serodiagnosis of adenovirus infections.

¹ Visiting scientist sponsored by the Netherlands Foundation. Present address: St. Elizabeth Hospital, Tiiburg, The Netherlands.