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Appl Environ Microbiol. 1971 April; 21(4): 723-725
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Semi-Micro, Dye-Binding Assay for Rabbit Interferon

John A. Armstrong

Department of Epidemiology and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

ABSTRACT

The semi-micro method for interferon assay was based on quantitation of inhibition of cytopathic effects in 6-mm wells. Cultures were exposed to interferon dilutions, challenged with vesicular stomatitis virus, and stained with methylrosanaline chloride. The bound dye was then eluted and measured colorimetrically. The method was more sensitive than the plaque-inhibition method and was extremely economical. Culture age was the most important factor affecting reproductibility.

Appl Environ Microbiol. 1971 April; 21(4): 723-725
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Copyright © 1971 by the American Society for Microbiology. All rights reserved.