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Appl Environ Microbiol. 1971 April; 21(4): 739-742
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

T-2 Toxin Production by Fusarium tricinctum on Solid Substrate ¹

Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

A method has been developed to produce and purify gram quantities of T-2 toxin [4ß, 15-diacetoxy-8-(3-methylbutyryloxy)-12, 13-epoxytrichothec-9-en-3-ol], a mycotoxin elaborated by a strain of Fusarium tricinctum isolated from toxic corn. After growing for 3 weeks at 15 C on 1,200 g of white corn grits, F. tricinctum NRRL 3299 elaborated at least 9.0 g of T-2 toxin, and 2.3 g of crystalline product was recovered. A lesser amount of toxin was produced on rice, but none was detected in wheat incubated at 20 C. The amount of toxin measured in white corn grits declined as the incubation temperature was raised to 20, 25, and 32 C.

¹ A preliminary report of this work was presented at the 70th Annual Meeting of the American Society for Microbiology, Boston, Mass., 26 April-1 May 1970 (Bacteriol. Proc., 1970, p. 14).