This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stancek, D. Articles by Paucker, K. Search for Related Content PubMed Articles by Stancek, D. Articles by Paucker, K. Agricola Articles by Stancek, D. Articles by Paucker, K.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 June; 21(6): 1067-1071
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Preparative Electrophoresis of Isotopically Labeled L-Cell Interferons ¹

Daniel Stanek² and Kurt Paucker

Department of Microbiology, The Medical College of Pennsylvania, Philadelphia, Pennsylvania 19129

ABSTRACT

The preparative method of polyacrylamide gel electrophoresis was adapted for purification and characterization of isotopically labeled L-cell interferons. Re-covery of interferon activity was quantitative, and purification and resolution were comparable to those obtained by analytical polyacrylamide gel electrophoresis. Ultimate specific activities attainable ranged from 2 x 10⁶ to 3 x 10⁶ international units per mg of protein.

² On leave of absence from the Institute of Virology, Czechoslovak Academy of Sciences, Bratislava, Czechoslovakia.

¹ This investigation was performed at the Virus Laboratories of The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pa.