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Appl Environ Microbiol. 1971 September; 22(3): 325-328
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Two-Tube Test for the Rapid Identification of Prompt Lactose-Fermenting Genera Within the Family Enterobacteriaceae

Department of Microbiology, The Haukeland Hospital, Bergen, Norway
The Gade Institute, Bergen, Norway

ABSTRACT

One hundred and fifty-nine prompt lactose-fermenting strains of Enterobacteriaceae were tested for H₂S production, ornithine decarboxylase activity, motility, and citrate utilization by using two different sets of test media. It was shown that the three tests first mentioned could be carried out in a single tube, the results being as reliable as when each was done separately. Simmons medium was preferred for the detection of citrate utilization. Since these four tests require only two tubes, they are considered more convenient in routine diagnostics. A combined medium for the detection of H₂S production and motility is described.