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Appl Environ Microbiol. 1971 September; 22(3): 459-462
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Simultaneous Use of Contrasting Fluorochromes to Separate Measles and Canine Distemper Viruses in a Common System

Veterinary Biologics Division, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010

ABSTRACT

Measles and canine distemper viruses were grown together in a Vero monkey kidney cell line. Each virus could be identified and individually titrated by using the color contrast produced by the reddish tetra-methyl rhodamine isothiocyanate-tagged antimeasles conjugate and the green fluorescein isothiocyanate-tagged antidistemper conjugate. Both blue light and green light were used for the excitation of the fluorochromes. Incident light was transmitted to the specimen by a vertical illuminator of the Ploem type.