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Appl Environ Microbiol. 1971 October; 22(4): 534-537
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Improvements in the Growth of BHK-21 Cells in Submerged Culture

The Animal Virus Research Institute, Pirbright, Surrey, England

ABSTRACT

A medium is described which will support the submerged culture of BHK-21 cells to 6.5 x 10⁶ to 7 x 10⁶ cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 x 10⁶ to 7 x 10⁶ cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.

¹ Present address: The Wellcome Laboratory, Pirbright, Surrey, England.