This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Read, W. C. S. Articles by Read, J. J. Search for Related Content PubMed Articles by Read, W. C. S. Articles by Read, J. J. Agricola Articles by Read, W. C. S. Articles by Read, J. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 November; 22(5): 769-771
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Estimation of the Growth of the T₁ Strain of Mycoplasma mycoides in Tryptose Broth by the Measurement of Lactate Dehydrogenase

II. Application to Vaccine Production

East African Veterinary Research Organization, P.O. Kabete, Kenya

ABSTRACT

The measurement of lactate dehydrogenase activity has been used to evaluate the growth of the T₁ vaccine strain of Mycoplasma mycoides var. mycoides in broth during the production of vaccine. Estimations were made during incubation at 37 C and storage at 4 C. The results were compared with titers of the vaccine obtained by two conventional methods of assessing viability titers: the counting of colonies formed on solid medium and a 50% end point titer found after dilutions were made in broth. The method of measuring lactate dehydrogenase activity allowed the rapid enumeration of organisms during incubation of the culture, but estimations made on vaccine stored at 4 C did not compare well with the two conventional methods of assessing viability. The lactate dehydrogenase activity of the culture has enabled rapid monitoring of the production stages of this vaccine.

¹ On secondment from: Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K.