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Appl Environ Microbiol. 1971 November; 22(5): 856-861
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Clostridium perfringens

I. Sporulation in a Biphasic Glucose-Ion-Exchange Resin Medium

Microbiology Division, Food Laboratory, U.S. Army Natick Laboratories, Natick, Massachusetts 01760

ABSTRACT

A biphasic culture medium suitable for cultivation and sporulation of Clostridium perfringens, C. botulinum, and C. sporogenes was devised. The medium designed for use in a disposable, compartmented, plastic film container contained peptones, yeast extract, minerals, an anion exchange resin, and glucose in 4% agar as the solid phase and (NH₄)₂SO₄ and 0.1% agar as the liquid phase. With the biphasic system, it was not necessary to use active cultures as inocula. Growth was at least equal to that obtained in conventional media, and spore production of 9 out of 12 strains of C. perfringens equalled or usually exceeded that of conventional media.

Present address: Da Nang, South Vietnam.