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Appl Environ Microbiol. 1971 November; 22(5): 925-927
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Preparation of Type 2 Herpes Simplex Virus Complement-Fixing Antigen

Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, Atlanta, Georgia 30333
U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

ABSTRACT

Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 M glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.