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Appl Environ Microbiol. 1971 December; 22(6): 1085-1090
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Metabolism of Pyrimidine Nucleotides in a Microorganism

III. Enzymatic Production of Ribose-5-Phosphate from Uridine-5'-Monophosphate by Pseudomonas oleovorans

Department of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan
Chemical Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

A study was made to develop a new method for the production of ribose-5-phosphate (R-5-P) from uridine-5'-monophosphate (UMP) by the action of nucleotide-N-ribosidase of Pseudomonas oleovorans, and a suitable medium for the formation of nucleotide-N-ribosidase was established. For the enzymatic conversion of UMP to R-5-P, a cell suspension was employed as the enzyme source. Although degradation of R-5-P, the desired product, occurred during the course of the enzyme reaction, it was prevented by the addition of an appropriate amount of zinc ion and resulted in a stoichiometric conversion of UMP to R-5-P and uracil. Accumulated R-5-P was readily isolated by ion-exchange chromatography of the bacteria-free reaction mixture. Yield of isolated R-5-P was about 60% of the theoretical recovery.

¹ Present address: Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Mozuumemachi, Sakai, Osaka.