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Appl Environ Microbiol. 1971 December; 22(6): 1123-1125
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Growth of Respiratory Syncytial Virus in Suspension Cell Culture

Alan V. Richman and Nicola M. Tauraso

Laboratory of Virology and Rickettsiology, National Institutes of Health, Bethesda, Maryland 20014
Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

Respiratory syncytial virus, Burnett strain, adsorbed efficiently and grew to high titers in suspension cultures of HEp-2 and MA-160 cells. Our results compared favorably with previous experience with the growth of respiratory syncytial virus in monolayer cell cultures. The use of suspension cell cultures provides a convenient and simple procedure for producing high-titering respiratory syncytial virus pools.


Appl Environ Microbiol. 1971 December; 22(6): 1123-1125
Copyright © 1971 American Society for Microbiology. All Rights Reserved.







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