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Appl Environ Microbiol. 1971 December; 22(6): 1141-1146
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Substituted Diazenes: Effect on the Growth of Enterobacteria and Possible Use as Selective Agents for Isolation of Pseudomonads

Meat Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19118

ABSTRACT

Incorporation of various diazenes into Trypticase soy media appeared selectively to permit the growth of pseudomonads while inhibiting the growth of a variety of enterobacteria. One of these diazenes, diamide (diazenedicarboxylic acid bisdimethylamide), was shown to be bactericidal for pure cultures of Escherichia coli, Proteus sp., and Salmonella enteritidis and to cause a 1- to 2-hr delay in the growth of Pseudomonas aeruginosa. When mixtures of these four organisms were inoculated into Trypticase soy broth or Trypticase soy agar (TSA) containing diamide, P. aeruginosa grew in overnight cultures. TSA containing diamide was also used successfully to isolate pseudomonads from soil, clinical urine specimens, fish, ground beef, ground pork, and ground veal.

¹ Present address: Division of Microbiology, Food and Drug Administration, Washington, D.C. 21204.

² Retired.