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Appl Environ Microbiol. 1971 December; 22(6): 987-991
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Production of L-Arginine by Arginine Hydroxamate-Resistant Mutants of Bacillus subtilis

Department of Applied Biochemistry, Tanabe Seiyaku Co. Ltd., Higashiyodogawa-ku, Osaka, Japan
Chemical Research and Development Laboratory, Tanabe Seiyaku Co. Ltd., Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

L-Arginine hydroxamate inhibited the growth of various bacteria, and the inhibition was readily reversed by arginine. L-Arginine hydroxamate (10^–3M) completely inhibited the growth of Bacillus subtilis. This inhibitory effect was prevented by 2.5 x 10^–4ML-arginine, which was the most effective of all the natural amino acids in reversing the inhibition. L-Arginine hydroxamate-resistant mutants of Bacillus subtilis were isolated and found to excrete L-arginine in relatively high yields. One of the mutants, strain AHr-5, produced 4.5 mg of L-arginine per ml in shaken culture in 3 days.