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Appl Environ Microbiol. 1972 January; 23(1): 141-144
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Electron Microscope Methods for the Identification of Adenoviruses Isolated in Micro Tissue Cultures¹

Virology Division, Naval Medical Research Unit No. 4, Great Lakes, Illinois 60088
Veterans Administration Hospital, Wood, Wisconsin 53193
Department of Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53232

ABSTRACT

A tissue culture micromethod is described for adenovirus isolation and preparation for presumptive identification by electron microscopy. These procedures are easier, more economical, and faster than conventional methods. The micro techniques make it more feasible to utilize direct visualization of virus in infected cells as an adjunctive diagnostic and research tool.

¹ This investigation was done in connection with research project MF12.524.009-4019AF61 of the Bureau of Medicine and Surgery, Navy Department, Washington, D.C.