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Appl Environ Microbiol. 1972 January; 23(1): 145-154
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Characterization of the Growth of Herpes Simplex Virus in Human Lymphoid Cells

^a Public Health Research Group, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14203

ABSTRACT

Herpes simplex virus was grown in a 6-liter suspended culture of an atypical permanent human lymphoid cell line, Roswell Park Memorial Institute no. 8226. The kinetics of virus replication were determined by counting viruses by electron microscopy, plaque formation, and tissue culture infectivity. Deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase activity was determined during the course of infection. Electron microscopy studies substantiated the kinetics of the virus infection in lymphoid cells.

¹ Present address: Israel Institute for Biological Research, Ness Ziona, Israel.