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Appl Environ Microbiol. 1972 January; 23(1): 155-157
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Disruption of Chemically Killed Bacterial Cells by a Synthetic Zeolite

^a Department of Microbiology, School of Medicine, University of Southern California, Los Angeles 90033

ABSTRACT

The applicability of a synthetic zeolite (type 4A, Union Carbide Corp., Linde Div., New York, N.Y.) as a disruptive agent in a procedure for the preparation of pure bacterial cell wall fractions from a variety of phenol-killed gram-negative, gram-positive, and acid-fast bacteria was demonstrated. The disruptive effect was found to be limited with formaldehyde-killed gram-positive cells and most gram-positive cocci killed either by phenol or formaldehyde.

¹ Bacteriology Laboratory, St. Mary's Hospital, Long Beach, Calif.

² Division of Biological Sciences, Bacteriology Section, University of Southern California, Los Angeles, Calif. 90007