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Appl Environ Microbiol. 1972 April; 23(4): 721-724
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Stable Reagent for the Detection of Antibody to the Specific Fraction I Antigen of Yersinia pestis1

James H. Rust Jr., Sanford Berman, William H. Habig, John D. Marshall Jr. and Dan C. Cavanaugh

Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012
Microbiology Division, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701

ABSTRACT

A stable hemagglutinating antigen for detection of fraction I (FR-I) antibody of Yersinia pestis (Pasteurella pestis) is described. The antigen was prepared by sensitizing tanned, pyruvaldehyde-treated sheep erythrocytes (PAT SRBC) with FR-I antigen. Preliminary standardization by titration of each lot of FR-I was required to minimize the effect of molecular heterogeneity of specific FR-I antigen and to eliminate nonspecific reactions caused by the presence of a minor antigenic contaminant. In tests with sera from rabbits, dogs, and humans, FR-I PAT SRBC were as reactive as the previously employed standard antigen, FR-I-sensitized tanned erythrocytes. Fluid suspensions of FR-I PAT SRBC stored at 4 C for 3 months, or lyophilized preparations stored at ambient temperature for 6 months, showed no loss in antigenic activity.

FOOTNOTES

1 A portion of this work will be included in a dissertation to be presented by Dan C. Cavanaugh to the Graduate Faculty of the Microbiology Department, University of Maryland, College Park.

Appl Environ Microbiol. 1972 April; 23(4): 721-724
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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