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Appl Environ Microbiol. 1972 April; 23(4): 758-764
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Effect of L-Aspartic Acid and L-Glutamic Acid on Production of L-Proline

Jyoji Kato, Masahiko Kisumi and Ichiro Chibata

Department of Applied Biochemistry, Chemical Research Laboratory, Tanabe Seiyaku Co., Ltd., Kashimacho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

To elucidate the effect of aspartic acid on growth of Kurthia catenaforma during the proline fermentation, this organism was compared with other bacteria with respect to the rate of consumption of aspartic acid, and to the activities of enzymes concerned in the metabolism of aspartic acid. Although no marked difference in enzyme activities was observed, the aspartic acid consumption rate of K. catenaforma was markedly higher than that of other organisms. The consumption of glutamic acid by K. catenaforma was not detected at 24 hr of culture. The difference between the consumption of aspartic acid and glutamic acid in this strain might result from a difference in permeability to the amino acids. We considered that L-glutamic acid might substitute for L-aspartic acid if the uptake of glutamic acid could be increased. A number of detergents were screened for their effect on consumption of glutamic acid. Cetyltrimethylammonium bromide, sodium laurylphosphate, and polyoxyethylene sorbitan monolaurate were found to increase the transport rate of glutamic acid, but not of aspartic acid. A method of producing L-proline from glutamic acid was established with the aid of detergents.

Appl Environ Microbiol. 1972 April; 23(4): 758-764
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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