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Appl Environ Microbiol. 1972 April; 23(4): 780-783
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Microculture Plaque Assay for Human and Simian Cytomegaloviruses

^a Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

ABSTRACT

The plaque assay for human and simian cytomegaloviruses routinely carried out in 60-mm petri dishes (macrocultures) has been adapted for use in microcultures in flat-bottom 16-mm circular wells of disposable plastic trays. Virus titrations and serum neutralization assays carried out in microcultures yielded reproducible results that were identical to those obtained in macrocultures.

¹ On leave from Sapporo Medical College, Sapporo, Japan, as a trainee of the United States-Japan Cooperative Medical Science Program.