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Appl Environ Microbiol. 1972 April; 23(4): 791-795
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Large-Scale Production of Lambda Bacteriophage and Purified Lambda Deoxyribonucleic Acid

^a Chemical Research Division, Hoffmann-La Roche Inc., Nutley, New Jersey 07110

ABSTRACT

Large amounts of a heat-inducible phage lambda mutant (CI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken DNA molecules.

¹ Present Address: Roche Institute of Molecular Biology, Nutley, N.J. 07110.