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Appl Environ Microbiol. 1972 April; 23(4): 814-818
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Laboratory Mill for Pulverizing and Homogenizing Nail Specimens as an Aid to Microscopy and Culture Confirmation of Onychomycosis

Department of Microbiology, and Pharmaceutical Research and Development, Schering Corp., Bloomfield, New Jersey 07003

ABSTRACT

A small mill has been developed for reducing nail clippings to a convenient size for microscopy and inoculation onto isolation media. The milling process acts to pulverize and homogenize the specimen. The use of a homogeneous sample in microscopy increases the opportunity for the discovery of fungal hyphae in a specimen. The use of a pulverized specimen increases the possibility of culture isolation by permitting greater numbers of potentially viable fungal cells to come into direct contact with the isolation medium.