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Appl Environ Microbiol. 1972 April; 23(4): 837-838
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Simplified Storage and Retrieval of Stock Cultures

Janice G. Nagel and Lawrence J. Kunz

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02114
Francis Blake Bacteriology Laboratories, The Massachusetts General Hospital, Boston, Massachusetts 02114

ABSTRACT

Multiple replicate units of stock cultures of certain bacteria and yeasts can be prepared, stored, and retrieved for use with a minimum of effort. The method involves the coating of sterile glass beads with a mixture of equal parts of broth culture and horse blood, and storage of the coated beads in a single, labeled, plastic tube in a mechanical freezer.

Appl Environ Microbiol. 1972 April; 23(4): 837-838
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.