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Appl Environ Microbiol. 1972 May; 23(5): 841-845
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Preparation of Purified Suspensions of Coxiella burneti by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation¹

Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012
Department of Biology, Catholic University, Washington, D.C. 20017

ABSTRACT

A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.

¹ A portion of these results are taken from a dissertation submitted to the Catholic University of America by D.R.D. in partial fulfillment of the requirements for an M.S. degree.