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Appl Environ Microbiol. 1972 May; 23(5): 875-879
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Simple Dye Release Assay for Determining Cellulolytic Activity of Fungi

Raymond P. Poincelot and Peter R. Day

Department of Biochemistry, The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504
Department of Genetics, The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504

ABSTRACT

A colorimetric assay, based on the release of dye from a paper substrate, is described as a simple and rapid means of detecting cellulolytic activity. It is readily adaptable to rapidly screening in situ large numbers of fungal liquid cultures. Since it is a nondestructive assay, samples can be recovered for later use.

Appl Environ Microbiol. 1972 May; 23(5): 875-879
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Copyright © 1972 by the American Society for Microbiology. All rights reserved.