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Appl Environ Microbiol. 1972 May; 23(5): 889-893
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Use of Gas Chromatography for Determining Catabolic Products of Arginine by Bacteria

Center for Disease Control, Atlanta, Georgia 30333

ABSTRACT

A rapid and sensitive procedure for determining catabolic products of arginine metabolism by bacteria was developed. The method consists of inoculating a solution of L-arginine with a heavy cell suspension of the test organism. After a 2-hr incubation period, dissimilation products (citrulline, ornithine, agmatine, putrescine) are converted to volatile derivatives and analyzed by gas-liquid chromatography. Compared with conventional microbiological tests, the new procedure is rapid and can be used for sensitive quantitative measurements of specific metabolites from arginine.