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Appl Environ Microbiol. 1972 May; 23(5): 919-922
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Quantitative Urine Culture Method Using a Plastic "Paddle" Containing Dual Media

Veterans Administration Hospital, Madison, Wisconsin 53705
Department of Medicine, University of Wisconsin School of Medicine, Madison, Wisconsin 53706

ABSTRACT

A new dip-inoculum method for quantitative urine culture is described which utilizes a dual-chambered plastic "paddle" housing both a general purpose and differential medium. Comparative bacterial counts of 1,000 clinical specimens using the pour plate and this device were identical in 82.9% and within a factor of five in 95.6%. The "paddle" detected all but 19 of 258 specimens (92.6%) with 100,000 or greater colonies per ml. This simple, convenient method should allow more extensive use of quantitative urine culture in the diagnosis and follow-up of patients with urinary tract infections in office practice. It should not be considered as a substitute for the more definitive pour plate method or for standard methods for characterization of bacteriological species when more exact information is required.