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Appl Environ Microbiol. 1972 May; 23(5): 923-930
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

Molecular Anatomy Program and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830
Northeastern Forest Experiment Station, Hamden, Connecticut 06514
Pacific Northwest Range and Experiment Station, Corvallis, Oregon 97331

ABSTRACT

Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 10¹³ polyhedral inclusion bodies per day. Continuous-flow methods for S- type purification have been evolved to deal with mass isolation of bioparticles.