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Appl Environ Microbiol. 1972 May; 23(5): 972-975
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

High-Intensity Vapor Lamp for Biological Research

^a Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

ABSTRACT

A vapor arc light source has been adapted to the study of the lethal action on bacteria of near-ultraviolet (UV) and visible light. Its use makes possible much shorter exposure times than could be obtained from previously available sources. The output of radiant energy is sufficient to provide a fairly detailed action spectrum for lethality in the long-UV and visible region without the addition of exogenous sensitizers. Populations of cells of Escherichia coli WP2 were inactivated through five log₁₀ cycles with light at 460 nm. Significant inactivation also was obtained with light at 550 and 650 nm.

¹ Present address: Department of Microbiology, University of Chicago, Chicago, Ill.